RNA

Part:BBa_K3201005:Design

Designed by: Nikolaos Karvelas, Anastasios Galanis   Group: iGEM19_Athens   (2019-10-15)


S.mansoni Type 1 Hammerhead Ribozyme, Cpf1 Optimised


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added a few bases so that a 5'-TTTN-3' sequence formed. This is necessary for Cpf1 to bind to the target DNA.


Source

S. mansoni genome (altered)

References

1.Klauser, B., Atanasov, J., Siewert, L. K. & Hartig, J. S. Ribozyme-Based Aminoglycoside Switches of Gene Expression Engineered by Genetic Selection in S. cerevisiae. ACS Synth. Biol. 4, 516–525 (2015). 2.Moon, S. B. et al. Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang. Nat Commun 9, 1–11 (2018).